Mechanisms of Gene Regulation
Jung, Eun-Jung , Jeong, Young-Min , Kim, Hoyeun , Lee, So-Young , Kim, Sang-Gu .
Roles of the first introns on the expression of Arabidopsis (Arabidopsis thaliana) genes for actin and actin-binding proteins.
We have recently reported intron-mediated enhancement in two actin-binding proteins (ABPs), Actin-Depolymerizing Factor (ADF) and profilin. To examine whether the expression of their target gene, actin, is also regulated by introns, we examined the role of the first intron on the expression of ACT2 gene, which encodes vegetative actin, using transgenic plants and a transient expression system. Transgenic plants carrying the ACT2 promoter-GUS with its first intron showed strong expression throughout the vegetative tissues, and the intron was needed for expression in the shoot apical meristem region and root tips. Along with changes in expression pattern, the intron also increased GUS expression about 10-fold. However, this enhancement was not observed when the intron was moved upstream of the ACT2 promoter or minimal promoter, suggesting that the intron is not a transcriptional enhancer. To further examine the role of the intron in the expression of actin and ABP genes, introns of actin, ADF, and profilin genes were exchanged. Introns of vegetative profilin and the ADF genes significantly enhanced gene expression under the control of heterogeneous promoters. Together with the role of the first intron of the ACT2 gene, these results suggest that the expression of actin and ABPs are regulated by a common mechanism.
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1 - Seoul National University, Department of Biological Sciences, Shillim-dong, Kwanak-gu, Seoul, 151-742,, Republic of Korea
2 - Seoul National University, Department of Biological Sciences
3 - Seoul National University, Department of Biological Sciences,
actin depolymerizing factor
intron mediated enhancement
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM