Protein Targeting and Vesicular Trafficking
Park, Hee Jin , Peer, Wendy , Murphy, Angus .
Localization and function of beta adaptin in Arabidopsis thaliana.
M1 aminopeptidases have shown to regulate the cellular trafficking mechanism. One member of the M1 family, AtAPM1 binds the auxin efflux inhibitor, NPA and has been shown to regulate the trafficking of auxin efflux proteins. When purified from Arabidopsis seedlings, APM was found to be associated with protein complexes containing beta adaptins associated with vesicular trafficking. Adaptor proteins are required for clathrin assembly of clathrin-coated vesicles. There are four kinds, heterohetrameric adaptor proteins AP1-4, each of which consists of two large, one medium, and one small adaptins. Beta adaptins, one of large adaptins are considered as the common structural unit to bind clathrin. Although we assume that plant beta adaptins function analogously with mammalian beta adaptins in terms of localizing and binding to clathrin in corresponding AP complexes, this hasnít been experimentally demonstrated. To place beta adaptins with subcellular compartments, beta adaptins fused to fluorescent reporter proteins will be transiently expressed with suscellular organelle markers in tobacco leaf or/and Arabidopsis protoplast. Brefeldin A (BFA), an inhibitor of secretary pathway, wortmannin, an endocytic inhibitor, and tyrophostins, inhibitors of the receptor-mediated endocytosis will be used as well. To characterize the AP complexes each of the large adaptins (alpha/gamma/delta/epsilon) and beta adaptin will be cloned for yeast two hybridization and bimolecular fluorescence complementation. Finally, we will determine which AP complexes are associated with APM1.
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1 - Purdue University, Department of Horticulture and Landscape Architecture, 625 Agriculture Mall Dr, West Lafayette, IN, 47907, USA
2 - Purdue University, Department of Horticulture and Landscape Architecture
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM