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Abstract Detail


Reproductive Development

Monfared, Mona [1], Gasser, Charles [2].

BASIC PENTACYSTEINE Proteins, a Novel Family of Putative Transcription Factors in Arabidopsis thaliana.

A group of novel nuclear-localized proteins that share a conserved C-terminal DNA binding domain was recently identified. The C-terminal domain has a preponderance of basic amino acids and a characteristic pattern of five conserved Cysteine residues. The BASIC PENTACYSTEINE (BPC) proteins were identified by virtue of their interaction with a positive regulatory element in the promoter of INNER NO OUTER (INO), a major ovule development gene. The seven BPC proteins of Arabidopsis are expressed in distinct or overlapping patterns throughout the plant and bind selectively to GA dinucleotide repeats. They have homologs in vascular and non-vascular plants and can be divided into three classes based on sequence similarity outside of the conserved DNA binding domain. The wide expression pattern for the BPCs and the prevalence of GA dinucleotide repeats in the Arabidopsis genome suggest a role for the BPCs in multiple areas of plant development and function. We are exploring the biological role of the BPCs by analyzing plants with mutations in multiple BPC genes or plants in which BPC gene expression is suppressed by RNA interference. Analysis of over/ectopic expression of BPC genes may add further information on gene function. We are also exploring gene targets of BPCs using chromatin immunoprecipitation followed by hybridization onto promoter microarrays. Expression data, mutant analysis, and DNA target identification will allow us to define the roles of the BASIC PENTACYSTEINE proteins in Arabidopsis and plants in general.


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1 - University of California, Davis, Molecular and Cellular Biology, 1 Shields Avenue, Davis, CA, 95616, USA
2 - University of California, Davis, Molecular and Cellular Biology

Keywords:
none specified

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P28033
Abstract ID:981


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