Baldwin, Patrick , Bauerle, William L. .
The Construction of a Molecular Imprinting Tool in the Investigation of the Plant Stress Response Hormone Abscisic Acid.
Molecular Imprinting technology is a well establish chemical procedure for molecule recognition. The principal behind the procedure is the formation of a polymer complex around a specific target molecule. Once formed, the complex contains cavities with binding sites that are complementary to the shape and size of the target molecule. In addition to the high specificity, molecular imprinting technology is non-invasive and dynamic (environmentally inert, thermal and pH stable). Currently, we are developing molecular imprint technology for use in abscisic acid (ABA) studies. The molecular imprinting tool will allow us hormone specific detection to characterize ABA biosynthesis. Currently, we have developed a molecular imprint that binds ABA. Using UV spectrophotometry and the ABA-derived molecular imprints, our lab has detected ABA in a series of in vitro experiments. We are now testing analogs of ABA for their binding affinity in order to determine specificity of the ABA-derived imprints. The next phase of our work will focus on refining the detection system for the molecular imprints to encompass a fluorescence/chemiluminescence detection system (permitting remote picomole concentration quantification). The molecular imprinting tool will then be used to quantify and characterize the hormonal water stress response from the cellular to ecosystem level.
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1 - Clemson University, Horticulture, E-143 Poole Agricultural Center, Box 340319, Clemson, SC, 29634-0319, United States
2 - Clemson University, Horticulture
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM