Plant biotech & Risk Assessment
Fang, Shih-Hua , Tsai, Shu-Chen , Chang, Yu-Ming , Chen, Long-Fang O. .
Bio-safety evaluations on ipt(isopentenyl transferase gene) transgenic broccoli delaying in postharvest yellowing.
Broccoli is a highly perishable fresh vegetable and tends to turn yellowing when it is subjected to ambient temperature in 1 to 3 days. Our studies on the transformation of broccoli with cytokinin synthaseă€”ipt (isopentenyl transferase) gene together with senescence associated promoter(SAG) and NPTII gene have indicated that some ipt transformed lines did have the effect on the retarding of floret yellowing in harvested floral heads. Three T5 ipt transformation selfed-lines with good agronomic performance and showing the retarding effect on yellowing in harvested floral heads have been selected for biosafety evaluations. Currently, we have initiated the studies on 1) transgene stability, transgene activity and expression, the sequences of flanking regions of transgene integration, anti-biotic marker gene activity, copy numbers of transgene, 2) general, chemical and mineral compositions assay, 3) general field performance and 4) animal-feeding model tests for the possible toxicity, allergenicity and immunity tests. DNA microarray and 2-D proteomic profiles are also included for the exploring possible unintended effects arisen from these transgenic broccolis. Presently, no significant concern is noted on animal feed studies such as the immunal responses on the mucosa immunity, mitogen-responsive activities of T cells and B cells, the phagocytosis activity of macrophages in peripheral blood and the levels of antibodies, IgA and IgE, in serum and relative percentages of CD4+ helper T cells, CD8+ cytotoxic T cells and CD19+ B cells. However, differences in some general and chemical components are noted, whether the observed differences are within the nature variations of broccoli and/or the similar cultivated vegetable crops and year to year or location to location differences requires further investigations.
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Postharvest biotechnology and transgenic methodology
1 - School of Medicine, China Medical University, Taichung, Taiwan, Department of Microbiology and Immunology,
2 - Taiwan Agricultural Research Institute, Taichung, Taiwan, Department of Agri-biochemistry
3 - Taiwan Agricultural Research Institute, Taichung, Taiwan, Department of Horticulture
4 - Academia Sinica, Taipei, Taiwan (ROC), Institute of Plant and Microbial Biology,, 128, Section 2, Yan-Jao-Yen road, Nankang, Taipei, Taiwan, 11529, Taiwan ROC
GMO food safety
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM