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Abstract Detail


Mineral Nutrition

Wasaki, Jun [1], Maruyama, Hayato [2], Tanaka, Miho [2], Yamamura, Takuya [3], Shinano, Takuro [3], Ito, Susumu [3], Osaki, Mitsuru [2].

Differentiated functions of acid phosphatases in white lupin plants are suggested from expression analysis.

Growth of white lupin plants is more vigorous than that of other crop plants under phosphorus (P) deficient condition. One of secreted acid phosphatases (APases) designated as LASAP2, has been focused as a major player to enable P acquisition from organic P in the rhizosphere. We have also identified two other APase homologs from white lupin and designated as LASAP1 and LASAP3. In this study we investigated the roles of APase homologs based on their expression analysis.
Seedlings of white lupin were transferred to nutrient solution or rhizobox. The solution contained 0 or 65 µM P for 3 weeks, then separated into leaves, stems and roots. In a soil culture, soil was fertilized except for P, and the plants were grown until flowering stage, then separated into immature, developed and senescent leaves, flowers and ripening seeds. cDNA was synthesized from total RNA of each sample, then quantitative real time PCR was performed to determine expression level of LASAP1, 2 and 3.
Upregulation of LASAP1 by P was found in all tested organs, especially in roots. The amount of LASAP1 mRNA in senescent leaves was higher than in younger leaves. The result suggests that LASAP1 systemically contributes to translocation of P by hydrolysis of organic P. Expression of LASAP2 gene was specific in roots under P conditions, as well as our results in previous studies. mRNA for LASAP3 was most abundant in ripening seeds. Accumulation of LASAP3 mRNA was much in immature leaves, whereas it was less in older leaves, stems and roots. It was suggested that primary structure of LASAP3 was not only similar to phytase from soybean seeds and but also had a domain of inositol phosphate synthetase. Thus, LASAP3 may play a role for phytate synthesis and P accumulation by the reverse reaction of APase.


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1 - Hokkaido Univ., CRIS, N21W10, Kita-ku, Sapporo, 001-0021, Japan
2 - Hokkaido Univ., Graduate School of Agriculture
3 - Hokkaido Univ., CRIS

Keywords:
phosphatase
phosphorus deficiency
white lupin
LASAP2.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P11011
Abstract ID:967


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