Growth and Vegetative Development
Arellano, Jesús , Fuentes, Sara Isabel , Castillo-Espana, Patricia , Lara, Miguel , Hernandez, Gerogina .
Indirect organogenesis of common bean (Phaseolus vulgaris L.): callus induction and shoot regeneration.
Common bean, Phaseolus vulgaris, the most important grain legume of the Phaseolus genus, had been recalcitrant for genetic transformation mediated by Agrobacterium and this could be related to its low frequency of in vitro regeneration. P. acutifolius is the only Phaseolus species that had been successfully transformed by Agrobacterium tumefaciens; regeneration of transgenic plants was achieved through an indirect regeneration method (Dillen et al. 1997). There had been several reports of in vitro regeneration of different Phaseolus species, however most of these use direct organogenesis (Zambre, et al. 2001). The aim of this work is to establish an indirect organogenesis procedure for regeneration of different common bean cultivars that could be used to test A. tumefaciens mediated transformation. We induced callus formation from apical meristems (AM) and cotyledonary nodes (CN) derived from mature embryos of P. vulgaris by cultivation on MS/B5 media supplemented with different concentrations of 3 auxins (IAA, 2,4-D and NAA) combined with different concentrations of the cytokinins BA or TDZ. Callus formed was induced to shoot formation by eliminating the auxin and increasing the cytokinin concentration. Ten different P. vulgaris cultivars from Mesoamerican and Andean origin were tested. Best results, i.e. highest indirect regeneration frequency, were obtained with Carioca, Kentucky wonder and Olathe cultivars. References: Dillen W, De Clercq J, Goossens A,Van Montagu M, Angenon G. 1997. Agrobacterium-mediated transformation of Phaseolus acutifolius A. Gray. Theoretical and Applied Genetics. 94:151-158 Zambre M, Geerts P, Maquet A, Van Montagu M, Dillen W, Angenon G. 2001. Regeneration of fertile plants from callus in Phaseolus polyanthus Greenman. Annals of Botany. 88:371-377
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Center for Genomics Sciences
1 - Centro de Ciencias Genomicas, Genomica Funcional de Eucariotes, Av. Universidad s/n Col. Chamilpa, Apdo. Postal 565-A, Cuernavaca, Morelos, 62210, Mexico
2 - Centro de Ciencias Genomicas, Programa de Genomica Funcional de Eucariotes
3 - Centro de Investigacion en Biotecnologia, Botanica Estructural
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM