Carr, Tyrell , Torney, Valerie , Whitham, Steven .
Functional analysis of the FRNK motif in the Turnip mosaic potyvirus helper component proteinase (HC-Pro).
A major determinant of symptom expression in potyvirus-infected plants is the viral-encoded helper-component proteinase (HC-Pro). Previous studies have shown that potyvirus HC-Pro mutants that attenuated symptom expression were impaired in virus accumulation or movement. However, a mutation in the highly conserved FRNK motif of Zucchini yellow mosaic potyvirus (ZYMV) HC-Pro was found that uncoupled HC-Pro’s role in symptom expression from its role in virus accumulation and movement. Interestingly, when the same mutation was introduced into Turnip mosaic potyvirus (TuMV) HC-Pro it impaired viral accumulation and symptom expression. Since ZYMV and TuMV HC-Pro mutants differ in amino acids flanking the FRNK motif, this prompted us to examine whether these amino acids conditioned the different phenotypes. To interrogate this possibility, additional TuMV mutants were generated and tested for infectivity and pathogenesis and corresponding HC-Pro constructs were tested for RNA silencing suppression activity. In addition transgenic plants expressing wild-type or mutant HC-Pro were generated to examine the genetics and molecular requirements for the FRNK motif and flanking amino acids in symptom development and HC-Pro RNA silencing suppressor activity.
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1 - Iowa State University, Plant Pathology, 351 Bessey Hall, Ames, Iowa, 50011-1020, USA
2 - Iowa State University, Plant Pathology
Turnip mosaic virus
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM