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Abstract Detail


Cell Walls

Liepman, Aaron H. [1], Nairn, C. Joseph [2], Willats, William G T [3], Sorensen, Iben [3], Roberts, Alison [4], Keegstra, Kenneth [5].

Functional Genomic Analysis Supports Conservation of Function Among Cellulose Synthase-Like A Gene Family Members and Suggests Diverse Roles of Mannans in Plants.

Mannan polysaccharides are widespread among plants, where they serve as structural elements in cell walls, as carbohydrate reserves, and potentially perform other important functions. Previous work has demonstrated that members of the CslA family of glycosyltransferases from Arabidopsis, Cyamopsis tetragonolobus, and Populus trichocarpa catalyze β-1,4-mannan and glucomannan synthase reactions in vitro. Mannan polysaccharides and homologs of CslA genes appear to be present in all lineages of land plants analyzed to date. In many plants, the CslA genes are members of extended multi-gene families, however it is not known whether all CslA proteins are glucomannan synthases. CslA proteins from diverse land plant species, including representatives of the mono- and dicotyledonous angiosperms, gymnosperms, and bryophytes, were produced in insect cells and each CslA protein catalyzed mannan and glucomannan synthase reactions in vitro. Microarray mining and quantitative real-time RT-PCR analysis demonstrated that transcripts of Arabidopsis and Pinus taeda CslA genes display tissue-specific expression patterns in vegetative and floral tissues. Glycan microarray analysis of Arabidopsis indicated that mannans are present throughout the plant, and are especially abundant in flowers, siliques, and stems. Mannans are also present in chloronemal and caulonemal filaments of Physcomitrella patens, where they are prevalent at cell junctions and in buds. Taken together, these results demonstrate that members of the CslA gene family from diverse plant species encode glucomannan synthases, and support the hypothesis that mannans function in metabolic networks devoted to other cellular processes in addition to cell wall structure and carbohydrate storage.


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1 - Eastern Michigan University, Biology Department, 316 Mark Jefferson Building, Ypsilanti, Michigan, 48197, USA
2 - University of Georgia, Warnell School of Forestry and Natural Resources
3 - University of Copenhagen, Department of Plant Physiology
4 - University of Rhode Island, Department of Biological Sciences
5 - Michigan State University, Department of Energy Plant Research Lab

Keywords:
glycosyltransferase
cellulose synthase like gene
mannan
glucomannan
cell wall.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P17020
Abstract ID:912


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