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Abstract Detail


Gene Structure and Function

Salmaso, Marzia [1], Lucchin, Margherita [1].

Grapevine germplasm diversity revealed by stilbene synthase multigenic family.

Most studies of genetic variation within Vitis species to date are based on random markers, such as microsatellites, but the characteristics that enable the species or its varieties to occupy a given geographical range or area might depend on a limited set of genes. Thus, study of genetic diversity could benefit from targeting genes that exhibit ecologically relevant variation, rather than random markers. In particular, single nucleotide polymorphisms (SNPs) are the most frequent form of variation in many genes. Resveratrol is a stilbene with well-known health-promoting effects in human that is produced constitutively or accumulated as a natural component of resistance to fungal diseases in grapevine. The resveratrol (trans-3,5,4'-trihydroxy-stilbene) synthesis is catalyzes by the enzyme trihydroxystilbene synthase (STS) encoded by a multigenic family close to 25 members. In grapevine, more than 15 STS genes have been characterized from different cultivars but not representative of different isogenes. Here we report a study on systematic SNP identification of Vitis vinifera stilbene synthase genes, as they are characterized by a high rate of variability. The goal is to set up a molecular reference system to the functional characterization of grapevine cultivars based on SNP detection. The research is performed by studying five grape local varieties showing from high to low resveratrol production. After cloning and sequencing different members of the multigene family, haplotypes have been defined by aligning the sequences of the amplified fragments. The heterozygosity at each locus by treating each haplotype as an individual allele has been estimated and DNA variation based on the nucleotide diversity parameter has been estimated.


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1 - University of Padova, Department of Environmental Agronomy and Crop Production, viale Universit 16, Agripolis, Padova, 35020, Italy

Keywords:
stilbene synthase
SNP
Grapevine.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P71002
Abstract ID:898


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