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Abstract Detail


Hormone Biology

Koiwa, Hisashi [1], Ueda, Akihiro [2], Vikram, Meenu [2], Li, Pinghua [3], Feng, Yue [2], Kim, Se Won [6], Kang, Chang Ho [2], Kang, Jae Sook [4], Yun, Dae Jin [5], Bahk, Jeong Dong [6], Lee, Sang Yeol [5], Fukuhara, Toshiyuki [7], Staswick, Paul E. [4], Pepper, Alan E. [8].

The carboxyl-terminal domain phosphatase-like 2 is a regulator of the leaf auxin response in Arabidopsis thaliana.

More than 20 genes in the Arabidopsis genome encode proteins similar to phosphatases that act on the carboxyl-terminal domain (CTD) of RNA polymerase II. One of these CTD-phosphatase-like (CPL) proteins, CPL2, dephosphorylates CTD-Ser5-PO4 in an intact RNA polymerase II complex and contains a double-stranded (ds)-RNA-binding motif (DRM). Although CPL2 DRM did not bind to dsRNA in vitro, T-DNA insertion mutants that express CPL2 variants lacking either a part of (cpl2-1) or entire (cpl2-2) DRM exhibited severe phenotypic aberrations, and increased salt sensitivity. Microarray and RT-PCR analysis of transcripts in cpl2-2 seedlings revealed that several auxin-inducible transcripts including DR5-GUS, a prototypical auxin-responsive reporter gene, were aberrantly regulated in cpl2-2. The level of endogenous auxin and its conjugates, and root growth response to exogenous auxin were similar between wild type and cpl2-2. However, basal and auxin-induced DR5-GUS expression in leaf mesophyll protoplast was attenuated in cpl2-2. Overexpression of ARF transcription factors partially suppressed the auxin response of the cpl2-2 protoplasts. These results identify CPL2 as an upstream regulator of auxin-regulated transcription that affects expression of a subset of early auxin-responsive genes.


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Related Links:
http://aggie-horticulture.tamu.edu/faculty/koiwa.html


1 - Texas A&M University, Department of Horticultural Sciences, Mail Stop 2133, Texas A&M University, College Station, TX, 77843, United States
2 - Texas A&M University, Department of Horticultural Sciences
3 - Univ. Illinois, Urbana-Champagne, Plant Biology
4 - University of Nebraska, Lincoln, Department of Agronomy and Horticulture
5 - Gyeongsang National University, Environmental Biotechnology National Core Research Center
6 - Gyeongsang National University, Division of Applied Life Science (BK21 program)
7 - Tokyo University of Agriculture and Technology, Department of Applied Biological Sciences
8 - Texas A&M University, Department of Biology

Keywords:
CTD phosphatase
auxin
transcriptional regulation.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P35029
Abstract ID:890


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