McClair, Janelle , Zareh, Meena , Rus, Ana , Bezmalinovic, Gorjana , Brusslan, Judy .
Identification of High Light Control Regions in the ELIP1 Gene Promoter in Arabidopsis.
Early Light Inducible Proteins (ELIPs) are thykaloid-localized proteins known to be expressed in response to high light conditions. When plants are returned to low light, ELIPs are rapidly degraded by ATP-independent proteases. The highly conserved high light expression pattern of these proteins suggests they play a critical role in plant responses to high light stress. Our goal is to identify regions in the Arabidopsis ELIP1 promoter that are necessary for the induction of ELIP1 expression in response to high light exposure. We previously determined that a 216 bp region upstream of the transcription start site was critical for the induction of ELIP1 under high light conditions. Several Light Regulatory Elements (LREs) were identified within that region based on the plant cis-acting regulatory DNA elements database (http://www.dna.affrc.go.jp/PLACE/), including the GATA, CAAT, G-box, TATAbox5, MYBcore, GT1-consensus and GT1-like motifs. To study these elements, the ELIP1 promoter was fused with GUS, a reporter gene, which can be quantitatively or visually analyzed. Single mutations as well as combinations of two or three mutations in the GATA, CAAT, and G-Box elements have been generated. However, no significant difference in expression of ELIP1 was found in these mutants when compared with the wild type promoter. Currently single and double mutants of TATAbox5, MYBcore, GT1-consensus, and GT1-like are being generated and analyzed.
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1 - California State University Long Beach
2 - California State University Long Beach, Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM