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Abstract Detail


Plant-Pathogen Interactions

Zhu, Jin [1], Patzold, William L. [2], Tranel, Patrick J. [2], Clough, Steven [3].

Comparing gene expression profiles from herbicide and pathogen treated soybean.

Previous studies implicated a role for photosynthetic components in disease resistance (Allen et al 1999, Seo et al 2000, Zou et al 2005). Several papers suggested more specifically that inhibition of photosystem II (PSII) was involved in the hypersensitive response (Allen et al 1999, and Seo et al 2000). To learn more on how soybean defense against pathogens might involve PSII, we examined soybean transcriptional response to PSII interfering herbicides. Atrazine inhibits photosynthetic electron transport by competitively binding to the plastoquinone (QB) binding site of the D1 protein of PS II (Steinback et al 1981, Hess 2000). It’s generally believed that bentazon interacts at the same site of PSII but there is evidence that suggests the actual binding site of bentazon is different from that of atrazine (Nimbal et al 1996). In our current study, soybean (Glycine max cv. Williams 82) plants were sprayed with atrazine, bentazon, or water mist when the first trifoliate leaves were fully expanded. First trifoliate leaflets were sampled at 1, 2, 4, 8 hours after treatment. Microarrays of PCR products amplified from soybean cDNA were carried out to monitor the gene expression patterns to the herbicide treatments. Microarray data from herbicide-treated plants are being compared to expression data from pathogen-challenged plants. Fuzzy-K-means is used for clustering to compare the different gene expression patterns between herbicide and pathogen treatments. Furthermore, the effect of pre-treatment with herbicide on soybean’s response to pathogen is studied by measuring chlorophyll fluorescence and pathogen growth.


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1 - university of illinois, crop sciences, 230 NSRC, 1101 W. Peabody, Urbana, Illinois, 61801, USA
2 - University of Illinois, Crop Sciences
3 - university of illinois, crop sciences; USDA-ARS, National Soybean Research Center, University of Illinois

Keywords:
Soybean
disease resistance
Photosynthesis
herbicide.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P15040
Abstract ID:866


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