A Symposium in Honor of Sherwin Carlquist
Jansen, Steven , Pletsers, Annelies , Steppe, Kathy , Cnudde, Veerle , Masschaele, Bert , Choat, B , Sano, Yuzou , Pesacreta, Thomas , Connell, Simon .
Three-dimensional imaging of wood anatomical characters using X–ray computed microtomography (microCT) and atomic force microscopy (AFM).
High resolution X-ray computed tomography provides an easy and fast way for non-destructive imaging of wood. A multi-purpose open type X-ray microCT equipped with a Feinfocus X-ray source was used at Ghent University (http://www.ugct.ugent.be/ugct/) to scan the wood structure of Fraxinus americana, Ulmus americana, Laurus nobilis, and Betula alba. Mature wood samples of 5 mm in diameter were scanned over a length of 4 to 14 mm. The spatial resolution obtained was between 2.5 Âµm and 5.8 Âµm, allowing us to distinguish individual cells of all cell types. High performance software packages (Octopus and VGStudio) were used to reconstruct 2D and 3D images. Although these microCT images do not provide enough resolution to visualize minute features such as pits in secondary walls, they offer a promising way to obtain quantitative measurements of the vessel network. The atomic force microscope (AFM) was applied to examine the structure of intervessel pit membranes. Because this technique does not require staining, coating, drying, or high vacuum for operation, it can be used to study pit membranes under liquid solution, i.e. in their native condition. The utility of applying AFM-techniques for observations of pit membranes in wood is discussed and compared to SEM- and TEM-observations.
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International Association of Wood Anatomists
Centre for X-ray tomography
1 - Royal Botanic Gardens, Kew, Jodrell Laboratory, Richmond, Surrey, TW9 3DS, United Kingdom
2 - Royal Botanic Gardens, Kew, Jodrell Laboratory, Richmond, Surrey, TW9 3DS, United Kingdom
3 - Ghent University, Laboratory of Plant Ecology, Coupure Links 653, Ghent, B-9000, Belgium
4 - Ghent University, Department of Geology and Soil Science, Krijgslaan 281/S8, Ghent, B-9000, Belgium
5 - Ghent University, Institute for Nuclear Sciences (INW), Proeftuinstraat 86, Ghent, B-9000, Belgium
6 - University of California, Davis, Department of Viticulture and Enology, 1 Shields Ave, Davis, California, 95616, USA
7 - Hokkaido University, Graduate School of Agriculture, Laboratory of Woody Plant Biology, Sapporo, 060-8589, Japan
8 - University of Louisiana at Lafayette, Microscopy Center, P.O. Box 42451, Lafayette, LA, 70504, USA
9 - University of Leeds, Institute for Molecular Biophysics, School of Physics and Astronomy, Leeds, LS2 9JT, United Kingdom
Presentation Type: Symposium or Colloquium Presentation
Location: Continental B/Hilton
Date: Tuesday, July 10th, 2007
Time: 8:45 AM