Abstract Detail
Water Relations Lee, Mi Ok [1], Hong, Choo Bong [2]. Cloning and characterization of glycine-rich RNA binding protein gene from Nicotiana tabacum. A cDNA clone encoding a glycine-rich RNA-binding protein was isolated from a cDNA library constructed from flooding-stressed tobacco. The cDNA was designated as NtGRP (Nicotiana tabaccum Glycine-rich RNA-binding Protein). The deduced amino acid sequence consists of 157 amino acids with an N-terminal RNA-recognition motif (RRM) and C-terminal glycine-rich domain. Comparative sequence alignment of NtGRP showed extensive homology to known glycine-rich RNA-binding proteins from other plant species. Genomic Southern blot analysis suggests that this gene exist as a single copy in N. tabaccum genome. Transcripts level of NtGRP was markedly increased by cold stress. And also increased by drought, salt and ABA treatment. Nucleic acid binding assay determined that NtGRP binds to ssDNA, dsDNA and mRNA. Ribopolymers RNA binding assay revealed that NtGRP shows high affinity for poly(G) and poly(A). Expression pattern, binding activity analysis and possible biological function of NtGRP are discussed.
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1 - Institute of Molecular Biology and Genetics, Department of Biological Sciences, Plant Molecular Physiology Lab, Seoul National University, seoul, 151-747, Korea
2 - Institute of Molecular Biology and Genetics, Department of Biological Sciences, Seoul National University
Keywords:
RNA-binding protein
glycien rich.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P09012
Abstract ID:845 | 