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Abstract Detail


Mechanisms of Gene Regulation

Umemura, Yoshimi [1], Hiratsu, Keiichiro [1], Ohme-Takagi, Masaru [1].

Molecular mechanism of the transcriptional repression mediated by a plant specific transcriptional repression domain, EAR-motif, in Arabidopsis.

We previously reported that the class II ERF transcription factors and a number of TFIIIA-type zinc-finger transcription factors are active repressors in plants. The repression domain of each factors is located in the C-terminal region that contains the conserved ERF-associated Amphiphilic repression (EAR) motif, which is essential for the activity of each repressors. The EAR-motif has been found only in plants. We utilize this EAR-motif for the novel gene silencing system (CRES-T), in which transcriptional activator was converted into a repressor by fusion with the EAR-motif to suppress the expression of the target genes (ref.1). Surprisingly, an amphiphilic motif composed of only 6 amino acids (XLxLXL) is sufficient for strong repression activity (ref.2). However, the mechanism of the repression mediated by the EAR-motif is still unknown. Because of the short peptide, the EAR-motif as itself does not appear to have enzymatic activity. We predict the EAR-motif may interact with other factor(s), such as co-repressor, to repress the transcription. We have isolated the factors that could interact with the EAR-motif using Yeast two-hybrid system. In this report we discuss the possible function of the factors and the mechanism of the EAR motif-dependent transcriptional repression.
References
1) Hiratsu et al. (2003) Plant J. 34, 733-739
Dominant repression of target genes by chimeric repressors that include the EAR motif, a repression domain, in Arabidopsis.
2) Hiratsu et al. (2004) Biochem. Biophys. Res. Commun. 321, 172-178
Identification of the minimal repression domain of SUPERMAN shows that the DLELRL hexapeptide is both necessary and sufficient for repression of transcription in Arabidopsis.


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Gene Regulation Research Group Web Site


1 - National Institute of Advanced Industrial Science and Technology(AIST), Research Institute of Genome-based Biofactory, AIST4, Higashi 1-1-1, Tsukuba, Ibaraki, 305-8562, Japan

Keywords:
transcriptional repression
EAR-motif
CRES-T
Yeast-two hybrid.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P36021
Abstract ID:838


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