| Abstract Detail
Plant biotech & Risk Assessment Lee, Kyeong-Ryeol [1], Kim, Myung-Sik [2], Lee, Ki-Jong [2], Park, In-Cheol [2], Lee, Seung-Beom [2], Kwon, Soon-Jong [3]. The stable inheritance and expression of cold shock protein gene introduced into tobacco chloroplast. The cold shock protein (CSP) gene was derived from Arthrobacter oxydans that isolated from Antarctic soil. Specially A2-5 strain of the species grow better than any other strain of that at 15°C. We cloned CSP gene from the strain by using PCR and introduced it into yeast. CSP transformed yeast can also grow well for 7 days in YPD medium at 15°C but wild-type yeast cannot grow. So we assumed that CSP functions as low temperature-resistant protein. In order to confirm if CSP plays a role in low temperature-resistance, we introduced CSP gene into tobacco chloroplast by using plastid transformation method. Southern blot analysis confirmed that the insertion of CSP gene in transgenic tobacco plant. Northern blot analysis showed that CSP gene was highly expressed in transgenic tobacco plant. The expression level is always high and stable at any condition, development stage and generation. In addition, Western blot analysis showed that the expression level of CSP was equivalent to that of RNA. To check if transferred DNA including CSP gene and spectinomycin resistance gene is located in chloroplast, transgenic tobacco lines were selected by antibiotics and reciprocal cross. When seeds of transgenic tobacco, reciprocal cross and wild-type were plated on the MS medium containing 0.5g/L of spectinomycin, all transgenic tobacco and reciprocal crossed tobacco that used wild-type as male parent grew well but wild-type and reciprocal crossed tobacco that used wild-type as female parent could not survive after germination. Although transgenic tobacco survive more than wild-type in the MS medium under -20°C for 45 min, it needs to treat freezing shock and low temperature for long time to transgenic tobacco in the soil.
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1 - National Institute of Agricultural Biotechnology, Division of Biosafety, 225 Seodundong, Suwon, Kyeonggido, 441-857, South Korea
2 - National Institute of Agricultural Biotechnology, Division of Biosafety
3 - National Institute of Agricultural Biotechnology
Keywords:
cold shock domain
low temperature
tobacco
Arthrobacter.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P45010
Abstract ID:814 |
