Unable to connect to database - 03:41:29 Unable to connect to database - 03:41:29 SQL Statement is null or not a SELECT - 03:41:29 SQL Statement is null or not a DELETE - 03:41:29 Botany & Plant Biology 2007 - Abstract Search
Unable to connect to database - 03:41:29 Unable to connect to database - 03:41:29 SQL Statement is null or not a SELECT - 03:41:30

Abstract Detail


Secondary Metabolism

Lo, Clive [1], Shih, CH [2], Chu, IK [3].

Quantitative analysis of anticancer 3-deoxyanthocyanidins in infected sorghum seedlings.

3-Deoxyanthocyanidins are structurally related to the anthocyanin pigments, which are popular as health-promoting phytochemicals. Here, it is demonstrated that the 3-deoxyanthocyanidins are more cytotoxic on human cancer cells than the 3-hydroxylated anthocyanidin analogues. At 200 microM concentration, luteolinidin reduced the viability of HL-60 and HepG2 cells by 90 and 50%, respectively. Sorghum is a major source of 3-deoxyanthocyanidins, which are present as seed pigments and as phytoalexins responding to pathogen attack. On the basis of the collision-induced dissociation spectra of luteolinidin and apigeninidin, an LC-MS/MS method, operating in multiple-reaction monitoring mode, was developed for the specific detection and accurate quantification of these compounds in complex mixtures, which may be difficult to analyze using absorbance measurements. The results demonstrated that inoculated sorghum seedlings could be utilized for convenient and large-scale production of 3-deoxyanthocyanidins. A quantity of almost 270 microg/g (fresh weight) of luteolinidin was produced 72 h after fungal inoculation of 1-week-old seedlings.


Log in to add this item to your schedule

1 - The University of Hong Kong, Dept of Botany, Pokfulam Road, Hong Kong, China
2 - The University of Hong Kong, Dept of Botany
3 - The University of Hong Kong, Dept of Chemistry

Keywords:
Sorghum 3-deoxyanthocyanidins
LC-SRM.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P20027
Abstract ID:809


Copyright 2000-2007, Botanical Society of America. All rights