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Abstract Detail


Molecular Biology

Husaini, Amjad Masood [1], Abdin, Malik Zainul [1].

High efficiency Agrobacterium tumefaciens-mediated transformation of Fragaria x ananassa Duch.: factors affecting transformation and regeneration of transgenic plantlets.

Following the previously described Agrobacterium tumefaciens mediated transformation procedures for Fragaria x ananassa Duch, we undertook several experiments to establish the importance of some parameters affecting transformation. High transformation efficiency of 10% was achieved using Agrobacterium strain GV2260 harbouring a binary vector (pBIN AR). The single most important factor that increased the percent recovery of transformants was the introduction of a preselection phase coupled with the addition of validamycin A, timentin and cefotaxime in the preselection regeneration medium. Introduction of a 7-day preculture phase improved transformation frequency by about two fold and cocultivation duration of 72-h seemed favourable for enhancing transformation efficiency. Transgene integration and copy number were assessed by PCR and Southern hybridization confirming single copy as well as multiple copies of transgene integration in 10 different lines of transgenic strawberry. Expression of osmotin gene was confirmed in transgenic lines TL3, TL5, TL9 using Northern hybridization.


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1 - Jamia Hamdard University, Department of Biotechnology, Hamdard Nagar, New Delhi, Delhi, 110062, India

Keywords:
Plant transformation
transgenic
strawberry
Agrobacterium
in vitro.

Presentation Type: Poster:Posters for Topics
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P73001
Abstract ID:80


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