Mitsuya, Shiro , Hattori, Tasuku , Fujiwara, Takashi , Tetsuko, Takabe .
Functional Analysis of Plasma Membrane Protein 3 (PMP3) Homologs in Higher Plants.
For plant salt tolerance, it is important to regulate the uptake and accumulation of Na+ ions. Although the function of PMP3 is not fully understood, it is proposed that PMP3 contributes to the restriction of Na+ uptake and consequently salt tolerance in yeasts. We have investigated function of PMP3 homologs in higher plants using Arabidopsis thaliana and a monocotyledonous halophyte Aneurolepidium chinense.1.We have found that disruption of RCI2A (PMP3 homolog in Arabidopsis thaliana) leads to over-accumulation of Na+ and increased salt sensitivity in Arabidopsis thaliana. RCI2A was suggested to play a role directly or indirectly for avoiding over-accumulation of excess Na+ and K+ ions in plants. These phenomenons were not observed with Ca2+ and Mg2+ ions.
2.We have found that overexpression of RCI2A decreases Na+ uptake and mitigates salinity-induced damages in Arabidopsis thaliana plants. The increase of malondialdehyde (MDA) and of H2O2 production caused by high salinity were greater in the shoots of wild type than in those of transgenic plants.
3.Then we were interested in PMP3 homologs in a monocotyledonous halophyte Aneurolepidium chinense (AcPMP3 genes). We have cloned AcPMP3-1 and -2 genes induced by salt stress. Interestingly the AcPMP3-1 gene was strongly induced in cells of the root cap and root epidermis, which strongly suggested that AcPMP3-1 is essential for regulating Na+/K+ transportation between plant roots and the outer environment under salt stress. We are planning to compare tissue specific expression of PMP3 homologs between Aneurolepidium chinense and salt sensitive rice in order to know if PMP3 gene expression manner determines the great difference in salt tolerance of both plants.
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1 - Nagoya University, Graduate School of Bioagricultural Sciences
2 - Nagoya University, Graduate School of Bioagricultural Sciences, Nagoya, 464-8601, Japan
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM