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Abstract Detail


Mechanisms of Gene Regulation

Kang, Kwon-Kyoo [1], Jung, Yujin [1], Arvin, Tulga [2], Dane, Fenny [3], Nou, Ill-Sup [4].

Isolation of Novel Chinese Cabbage (Brassica rapa L.) MAP Kinase Gene, BrMAPK3 Which Accumulates Rapidly in Response to Environmental Stresses in Transgenic Arabidopsis thaliana.

Mitogen-activated protein kinase (MAPKs) pathways are modules involved in the transduction of extracellular signals to intracellular targets in eukaryotes. In vertebrates, MAPKs are typically activated in response to various mitotic agents such as hormones and growth factors. Full length MAPK3 cDNA sequence (designated as BrMAPK3) was cloned and characterized for the first time from Brassica rapa, using RACE (rapid amplification of cDNA ends). The full-length cDNA of BrMAPK3 (1525bp) contains a 1428bp open reading frame (ORF) encoding a peptide of 475 amino acids with a calculated molecular mass of 52.25kD. In order to gain further insights into the expression/ regulation of BrMAPK3 gene, we characterized this activation against signaling molecules such as NaCl, Sucrose, CuSO4, UV irradiation and high and low temperatures. Here, we report that high (37 °C) and low (12°C) temperatures strongly induce mRNA levels of BrMAPK3 gene. Moreover, BrMAPK3 gene was introduced into Arabidopsis thaliana under the control of the cauliflower mosaic virus 35S promoter. The transgene was stably integrated and actively transcribed in transgenic plants. Transgene (BrMAPK3) was enhanced rapidly in response to low (4°C and 12°C) temperature in transgenic plants. Present results demonstrate dramatic transcriptional and transient regulation of the BrMAPK3 expression by abiotic stress, suggesting a role for BrMAPK3 in B. rapa stress response pathways.


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1 - Hankyong National University, Department of Horticulture
2 - Sunchon National University, Faculty of Plant Science and Production
3 - Auburn University, Department of Horticulture
4 - Sunchon National University, Faculty of Plant Science and Production, 315 Maegok-dong, Sunchon-si, Jeonnam-do, 540-742, Korea

Keywords:
kinase
abiotic and biotic stresses
B. rapa.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P36020
Abstract ID:783


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