Lawrence, Leslie , Settlage, Sharon , Hanley-Bowdoin, Linda .
Analysis of host and viral DNA replication components in plants.
Geminiviruses are single-stranded DNA viruses that infect plants. Because of increasing concern regarding the susceptibility of United States crops to these viruses, considerable research is being devoted to understanding and analyzing the mechanisms of geminivirus replication and infectivity. Dr. Hanley-Bowdoin’s lab is studying these processes using the geminivirus, Tomato golden mosaic virus (TGMV), as a model. My research project examined transgenic plants that express mutant versions of the geminivirus replication proteins, AL1 and AL3, that have the capacity to interfere with TGMV replication and confer resistance to infection. Several independent lines carrying the AL1/AL3 transgene show different resistant phenotypes. To understand the basis of these phenotypes, I have characterized expression of the mutant AL1 and AL3 proteins. For these experiments, I have used immunoblotting techniques to quantify viral protein expression in various tissues and developmental stages. I used immunohistochemical techniques to visualize the fraction of cells and their types that accumulate the viral proteins. Comparison of these results between different transgenic lines may provide insight into the basis of the different resistance phenotypes.
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1 - North Carolina State University, Molecular & Structural Biochemistry, Viral Genomics, Campus Box 7622, Raleigh, NC, 27695, USA
2 - North Carolina State University, Molecular & Structural Biochemistry
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM