Xu, Yan , Tian, Jiang , Huang, Bingru .
Root Protein Expression Associated with Thermotolerance of Geothermal and Turf-type Agrostis Grass Species.
Extensive research has focused on changes in protein metabolism of leaves in relation to plant thermotolerance. Limited information is available on protein changes associated with root thermotolerance. In this study, we examined the relationship of the expression pattern of heat shock proteins (HSP) in roots and thermotolerance for two Agrostis grass species contrasting in heat tolerance: heat-tolerant Agrostis scabra adapted to the geothermal soils and heat sensitive Agrostis stolonifera used as turfgrass. Roots of both species were exposed to increasing temperature (17, 24, 31, 38 and 45oC) and durations (2, 4, 24 and 48 h), while shoots were maintained at optimal growth temperature (17oC) in growth chambers. Total soluble protein content significantly decreased after 4 h at 45oC in both grass species and continued decreasing with prolonged treatment duration. Western blot analysis detected that constitutive HSP70 was consistently expressed after 2 h at all temperatures while inducible HSP70 started to accumulate after 4 h at temperatures above 24oC. HSP60 was highly induced at elevated temperatures after 4 h of exposure. A. scbara had stronger or earlier expression of HSPs than A. stolonifera, especially at lethal high temperatures (40 and 45oC). HSP17.6 (Class I) was primarily heat-induced, with enhanced expression with increasing temperatures and duration, particularly in heat-tolerant A. scabra. Our results suggested that HSP expression, particularly small molecular weight HSPs, may play important roles in controlling root thermotolerance in cool-season grass species.
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1 - Rutgers University, Plant Biology & Pathology, 59 Dudley Rd., New Brunswick, NJ, 08901, USA
2 - Rutgers Univeristy, Plant Biology & Pathology
3 - Rutgers University, Plant Biology & Pathology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM