Kaufman, Lon , Warpeha, K. M. , Upadhyay, S. , Yeh, J. , Adamiak, J. , Hawkins, S. I. , Lapik, Y. R. , Anderson, M.B. .
The GCR1, GPA1, PRN1, NF-Y signal transduction chain mediates both blue light and ABA responses in etiolated Arabidopsis.
Different classes of biotic (e.g. plant hormones) and abiotic (e.g. different wavelengths of light) signals act through specific signal transduction mechanisms to coordinate all of higher plant development. While a great deal of progress has been made, full signal transduction chains have not yet been described for most blue light, or abscisic acid-mediated events. Based on data derived from T-DNA insertion mutants, yeast two-hybrid and protein co-precipitation assays, we report a potential signal transduction chain, shared by blue light and ABA, which leads to Lhcb expression in etiolated Arabidopsis seedlings: GCR1 (the sole Arabidopsis protein coding for a potential G-protein coupled receptor), GPA1 (the sole Arabidopsis G-alpha subunit), PRN1 (Pirin1, one of four members of an iron-containing subgroup of the cupin superfamily), and an NF-Y heterotrimer. NF-Y factors are transcriptional regulators expressed in different tissues and at different developmental stages in higher plants. Each subunit of the heterotrimer has 9-10 copies in each gene family. Our data demonstrates that the signaling complex for Lhcb signaling is comprised of NF-Y-A5, B9 and possibly C9. We also demonstrate that this same mechanism of signaling is present in imbibed seeds where it affects germination rate and very early development, by using different possible combinations of NF-Y subunits.
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1 - University of Illinois at Chicago, Biological Sciences, 900 S. Ashland Ave, m/c 567, rm 4268, Chicago, IL, 60607, USA
2 - University of Illinois at Chicago, Biological Sciences
3 - University of Illinois at Chicago, Molecular Genetics
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM