Veerappan, Vijaykumar , Wang, Jing , Shi, Huazhong , Allen, Randy D. .
Genetic mapping and characterization of Arabidopsis mutants deregulated in GSTF8 expression.
Several mutants that are aberrant in GSTF8 expression were identified by utilizing EMS mutagenized transgenic Arabidopsis line carrying Arabidopsis GSTF8:luciferase promoter-reporter fusion. Among all the identified mutants, gce1, gce2 (GSTF8 constitutive expression), and ghes (GSTF8 hyper-responsive expression to salicylic acid) were selected for further physiological, molecular and genetic characterization. All three mutants are governed by single, recessive mutations and display defective morphological phenotypes. Allelism test between two different constitutive mutants gce1 and gce2 revealed that they are not allelic. Moreover, all three mutants show aberrant expression of GSTFs and defense-related transcripts. In an effort to identify the mutation responsible for mutant phenotype, mutants are outcrossed to an alternative ecotype Ler-O. Homozygous mutant plants were selected in F2 mapping population based on aberrant luciferase phenotype for genetic mapping. Scoring for recombination events in 600 F2 plants using SSLP and CAPS markers localized one of the mutant to an Arabidopsis chromosome at 100 Kb mapping interval. Further fine mapping and other approaches are underway to pinpoint the genetic lesion causing the mutant phenotype. Map-based cloning and further characterization of gce and ghes mutants will not only identify novel signal transduction components but also provide new insights into the molecular mechanisms underlying oxidative stress and plant defense responses in Arabidopsis.
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1 - Texas Tech University, Department of Biological Sciences, Lubbock, TX, 79409, USA
2 - USDA-ARS Cropping Systems Research Laboratory
3 - Departments of Chemistry and Biochemistry, Texas Tech University
4 - Texas Tech University, Departments of Biological Sciences and Plant and Soil Science
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM