Cross, Joanna , Collakova, Eva , Gehan, Jackson , DellaPenna, Dean , Shachar-Hill, Yair .
QTL Analysis of Fluxes in Developing Arabidopsis Embryos.
Arabidopsis thaliana is closely related to Brassica napus, a major oil crop. That and the available sequence, mapping and expression data make Arabidopsis an attractive model for oilseed crops. Given the many interconnections between different central metabolic pathways it is important to take a systems approach to improve our understanding of seed biochemistry. Accordingly, steady state flux analysis provides a general description of the flow through the metabolic network. This approach requires an experimental system that is relatively homogenous and physiologically stable. For that reason embryos are preferred over whole seeds. Seed development is a complex process under the control of multiple genes. Fortunately, natural variation is extensively found in Arabidopsis. Quantitative Trait Locus (QTL) mapping provides insight into traits under the control of several loci. The objective of this project is to perform QTL analysis on Arabidopsis developing embryos to pinpoint genes involved in modulating metabolic fluxes during the early stages of seed maturation. This requires (i) the selection of Arabidopsis ecotypes with different seed characteristics, (ii) the set-up of a flux map for the parental ecotypes, (iii) and finally the QTL analysis in about 100 Recombinant Inbred Lines (RIL). We will report on the progress accomplished in the following areas. A set of 20 ecotypes has been selected based on the availability of RILs. Labeling experiments will be performed to identify those individuals with the greatest flux differences. In addition, we are using available expression, localization and literature information to build a metabolic framework of the Arabidopsis embryo. Finally, culture conditions reproducing in vivo environments are being developed.
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1 - Michigan State University, Plant Biology, 166 Plant Biology Building, East Lansing, Michigan, 48824, USA
2 - Michigan State University, Plant Biology
3 - Michigan State University, Biochemistry and Molecular Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM