| Abstract Detail
Plant-Pest Interactions Mabood, Fazli [1]. Induction of pathogenesis-related proteins during biocontrol of Rhizoctonia solani with Pseudomonas aureofaciens in soybean (Glycine max L. Merr.) plants. To investigate the biocontrol effectiveness of antibiotic producing bacterium, Pseudomonas aureofaciens 63-28 against the phytopathogen Rhizoctonia solani AG-4 on Petri plates and in soybean roots, growth response and induction of PR-proteins were estimated after inoculation with P. aureofaciens 63-28 (P), with R. solani AG-4 (R), or with P. aureofaciens 63-28 + R. solani AG-4 (P + R). P. aureofaciens 63-28 showed strong antifungal activity against R. solani AG-4 pathogens in Petri plates. Treatment with P. aureofaciens 63-28 alone increased the emergence rate, shoot fresh weight, shoot dry weight and root fresh weight at 7 days after inoculation, when compared to R. solani AG-4; P+R treatment showed similar effects. Peroxidase (POD) and β-1,3-glucanase activity of P. aureofaciens 63-28 treated roots increased by 41.1 and 49.9%, respectively, compared to control roots. POD was 26% greater in P+R treated roots than R. solani treated roots. Two POD isozymes (59 and 27 kDa) were strongly induced in P+R treated roots. The apparent molecular weight of chitinase from treated roots, as determined through SDS-PAGE separation and comparison with standards, was about 29 kDa. Five β-1,3-glucanase isozymes (80, 70, 50, 46 and 19 kDa) were observed in all of treatments. These results suggest that inoculation of soybean plants with P. aureofaciens 63-28 elevates plant growth inhibition by R. solani AG-4 and activates PR-proteins, potentially through induction of systemic resistance mechanisms.
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1 - McGill Univeristy Montreal, Canada, Plant Science, 21,111 Lakeshore Road, Ste-Anne-De-Bellevue, Qc, h9x3v9, Canada
Keywords:
Rhizoctonia solani
Pseudomonas
Soybean.
Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P14014
Abstract ID:741 |
