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Abstract Detail


Mechanisms of Gene Regulation

Li, Chia-Wen [1], You, Su-Juan [2], Sanjaya, Sanjaya [3], Chan, Ming-Tsair [4].

Transgenic CBF1 tomato plants manifest enhanced tolerance to Ralstonia solanacearum via activation of LeERFs and pathogenesis-related (PR) genes.

Agricultural losses caused by phytopathogens are difficult to assess but estimated to be substantial and expected to increase with time. Here, we report the molecular mechanism involve in the pathogen tolerance of CBF1 transgenic tomato plants mediated by the expression of some pathogensis-related(PR) genes. Northern blot analysis indicated that the transcripts of these genes significantly accumulated in CBF1 transgenic tomato plants. Further, when transgenic plants challenged with Ralstonia solanacearum strain Pss4 (race 1, biovar 3) did not affect the growth, whilst wild-type plants wilted. Several ethylene-responsive factor (ERF) family genes are induced in CBF1 transgenic tomato confirmed by RT-PCR. Moreover, these ERFs can binds to GCC box and enhance promoter activity, suggesting that ERFs presumably may act as intermediate transcription factors in between CBF1 and PR genes in tomato under pathogen stress. Taken together, CBF1 regulate pathogen stress response in tomato and the interaction of different stress responsive genes through interacting with different cis-acting elements (GCC box), even other transcription factors


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1 - Academia Sinica, Agricultural Biotechnology Research Center, No.128 Sec.2 Academia Rd. Nankang, Taipei, 115, Taiwan
2 - Acadamia Sinica, Agricultural Biotechnology Research Center, No.128 Sec.2 Academia Rd. Nankang,, Taipei, 115, Taiwan
3 - Academia Sinica, Agricultural Biotechnology Research Center
4 - Academia Sinica, Agricultural Biotechnology Research Center

Keywords:
CBF1
Ralstonia solanacearum
ethylene response factor
Pathogenesis-related proteins.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P36019
Abstract ID:739


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