Unable to connect to database - 11:00:29 Unable to connect to database - 11:00:29 SQL Statement is null or not a SELECT - 11:00:29 SQL Statement is null or not a DELETE - 11:00:29 Botany & Plant Biology 2007 - Abstract Search
Unable to connect to database - 11:00:29 Unable to connect to database - 11:00:29 SQL Statement is null or not a SELECT - 11:00:29

Abstract Detail


Water Relations

Hachez, Charles [1], Zelazny, Enric [2], Muylaert, Mélanie [2], Cavez, Damien [2], Heinen, Robert [2], Veselov, Dmitry [2], Chaumont, François [2].

Plasma membrane aquaporins are widely expressed in maize and form heteromers to regulate plant water movement.

Water movement across plant tissues occurs by parallel apoplastic, symplastic and transcellular pathways. At the cell level, aquaporins (AQP) play an important role in regulating transmembrane water flow. Thirteen plasma membrane aquaporin (PIP) genes are expressed in maize. We quantified and localized PIP expression and activity in Zea mays roots and shoots at both RNA and protein levels (Hachez et al., 2006, Plant Mol. Biol. 62, 305-323 and unpublished data). Expression was found to be dependent on the developmental stage of the plant, with, in general, an increase in expression towards the elongation zone of both leaves and roots. This expression was also found to be diurnally regulated. In situ expression studies revealed that PIPs are expressed in cells where transmembrane water movement is required. The observation that several PIPs colocalized in cells suggests that they may interact to regulate the cell water permeability. ZmPIP heteromerization was further supported by oocyte swelling assays, immunofishing experiments and Förster resonance energy transfer (FRET) analysis (Fetter et al., 2004; Zelazny et al., submitted). Silencing of PIP isoforms is on the way and should provide important information about their physiological role in maize.


Log in to add this item to your schedule

1 - Universite catholique de Louvain, Institut des Sciences de la Vie, Croix du Sud 5-15, Louvain-la-Neuve, 1348, Belgium
2 - Universite catholique de Louvain, Institut des Sciences de la Vie

Keywords:
aquaporin
membrane protein
gene expression
root development
shoot
Water relations.

Presentation Type: ASPB Minisymposium
Session: M29
Location: Continental C/Hilton
Date: Wednesday, July 11th, 2007
Time: 11:55 AM
Number: M29004
Abstract ID:736


Copyright © 2000-2007, Botanical Society of America. All rights