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Abstract Detail

Secondary Metabolism

Wiberley, Amy E. [1], Donohue, Autumn R. [2], Sharkey, Thomas D. [2].

Use of Populus trichocarpa as a model species for hydrocarbon emission.

Isoprene is a biogenic hydrocarbon that has a substantial impact on tropospheric chemistry. It is emitted from many plant species, including a large number of trees. Isoprene is synthesized by isoprene synthase, from dimethylallyl diphosphate made by the methylerythritol 4-phosphate pathway. It has previously been demonstrated that isoprene emission in developing leaves is regulated at the level of transcriptional control of isoprene synthase, but molecular regulation of emission in mature leaves is largely unstudied. One study of mature leaves subjected to changes in growth temperature indicated that emission is controlled by dimethylallyl diphosphate supply in mature leaves; in other words, by the flux through the methylerythritol 4-phosphate pathway. Therefore, molecular tools are being developed to study the eight enzymes that comprise this pathway. These studies are being done in Populus trichocarpa, which is a prolific isoprene emitter; it and closely related species are very widely planted, increasing the importance of understanding its regulation of isoprene emission. It is uniquely suited to these studies, as it is the first emitting species to have a sequenced genome. Some of the newly developed tools for study of regulation of isoprene emission from Populus trichocarpa are presented here.

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1 - University of Wisconsin-Madison, Botany, 430 Lincoln Dr., Madison, WI, 53706, U.S.A.
2 - University of Wisconsin-Madison, Botany

methylerythritol 4-phosphate
dimethylallyl diphosphate
metabolic regulation.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P20002
Abstract ID:66

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