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Abstract Detail


Plant-Pathogen Interactions

Shrestha, Roshi [1], Wilson, Mike [2], Price, Adam [2].

PLANT NEMATODE INTERACTION: tolerance to root knot nematode in Oryza sativa.

Meloidogyne graminicola, rice root knot nematode is a major pest of rice in Asia and West Africa for which resistant or tolerant varieties are not currently available. Two varieties from two sub species of O. sativa (Japonica=Azucena and Indica=Bala) have been used to create a mapping population, which has been used extensively to detect the QTL for various agronomic traits. These two parent varieties were found to differ quantitatively in their susceptibility to M. graminicola. Experiments consistently showed that Azucena supports more than twice the number of galls compared to Bala and the galls on Azucena roots contain more than twice the number of juvenile nematodes. The total seed weight and root weight were significantly (P<0.05) reduced in the presence of nematodes only in Azucena. This indicates that Bala has a good tolerance to M. graminicola since nematodes can reproduce on this variety but do not impact on the plants ability to yield. In two experiments, gall numbers were estimated for 144-156 recombinant inbred lines two and four weeks after inoculation. A total of five and two significant or putative quantitative trait loci (QTLs) (LOD score ≥ 3.2 or ≥2.4 respectively) for nematode tolerance were detected in the two experiments. The QTL on chromosome 6 was detected in both experiments. For two of the QTLs detected, Azucena was the donor of the tolerant alleles, suggesting it will be possible to breed plants with greater tolerance than the more tolerant parent. QTLs detected could be used as the potential targets to be further characterised and be manipulated in molecular breeding.


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1 - University of Aberdeen, College of Life Sciences and Medicine, Cruickshank BUilding, St Machar Drive, Aberdeen, Scotland, AB24 3UU, UK
2 - University of Aberdeen, College of Life Sciences and Medicine

Keywords:
Oryza sativa
root knot nematode
Tolerance
Quantitative trait loci (QTL).

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P15034
Abstract ID:595


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