Qouta, Lolita , Abdel-Massih, Roula , Brett, Chris , Nimmo, Hugh , Baydoun, Elias .
Biochemical and immunohistochemical analysis of pectic polysaccharides in the cell walls of salt tolerant Arabidopsis suspension cultures.
Pectic polymers are highly complex in structural terms; they have been reported to play a distinct role in cell wall deposition, assembly, porosity, expansion and cell-cell adhesion that in turn determines the mechanical properties of the cell wall. Plants have a remarkable ability to cope with variable environmental stresses; high salinity causes pleiotropic effects in plant growth such as reduced cell expansion, decreased protein synthesis, and accelerated cell death. In a study of salt tolerant tobacco (Nicotiana tabacum) suspension cells, an increase of pectin and poly- GalA in the cell wall was observed by FT-IR microscpectroscopy (McCann et al., 1994).The present study investigates the differences in cell adhesion between wild-type and salt-tolerant Arabidopsis suspension cultures. Modifications in the cell wall composition and structure were investigated through in situ immunocytochemical analyses, along with the biochemical characterization of the pectic extracts and changes in pectin modifying enzymes during the proliferation of the culture.McCann M, Shi J, Roberts K, Carpita N (1994).The Plant Journal 5: 773–785.
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1 - Institute of Biomedical and Life Sciences, University of Glasgow, UK
2 - Department of Biology, University of Balamand, Lebanon
3 - American University of Beirut, Department of Biology, Beirut, Lebanon
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM