Li, Yan , Borevitz, Justin .
Natural Genetic Diversity and Population Structure of Arabidopsis thaliana in the U.S. Midwest and World-wide Collections.
Natural variation in Arabidopsis thaliana can be exploited to identify new alleles of known genes, new quantitative trait loci (QTLs), perform association mapping studies, and discover the ecological and evolutionary forces driving local adaptation and shaping the genetic diversity. We genotyped over 800 lines from the Arabidopsis Biological Resource Center (ABRC) plus over 900 lines collected from different locations in the U.S. Midwest across different times of year for 149 common SNPs to determine the relatedness and population structure. We observed different levels of genetic diversity among different local populations in the Midwest. Certain locations were clonal with identical genotypes at 149 common SNPs however extensive phenotypic variation was still present likely due to new mutations. Some locations contained multiple diverse strains, including new natural recombinant inbred lines. Certain common Midwest genotypes were identified across multiple collection locations. The possible ancestral origins of the U.S. Midwest populations will be inferred via model-based structure analysis with world-wide stock center collections. A maximal genetic diversity HapMap panel of 384 strains is being selected for high density genotyping with a custom 250kSNP/tiling array (AtSNPtile1) that will be used for whole genome association mapping. The high marker density, large population size, and species wide diversity will allow powerful LD mapping studies to reveal common variation important for adaptation.
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1 - University of Chicago, Ecology and Evolution, Chicago, IL, 60637, US
2 - University of Chicago, Ecology and Evolution, Committee on Genetics
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM