Do, Phuc Thi , Zuther, Ellen , Erban, Alexander , Heyer, Arnd Göran , Köhl, Karin , Kopka, Joachim .
Polyamine metabolism in response to drought and salt stress including comparative metabolome analysis of the salt response in rice.
Drought is the major problem in rain-fed rice production, as more than 50% of rain-fed lowlands are drought-prone. Additionally soil salinity is a major limiting factor in agricultural productivity and affects large terrestrial areas of the world. To investigate how polyamines contribute to drought and salt tolerance of rice, we studied 25 Indica cultivars from Vietnam and four Japonica cultivars including Nipponbare using a moderate long-term drought stress and a hydroponic culture system for salt stress. The cultivars could be classified as either tolerant, intermediate or sensitive based on visual inspection and photosynthetic parameters including Imaging PAM analysis. The role of polyamine metabolism in rice abiotic stress tolerance is subject to controversial discussions. To gain detailed information of changes in polyamine levels during drought and salt stress, we measured the soluble fraction of polyamines in leaves by HPLC and of compounds related to polyamine metabolism and stress by GCMS. Regulatory principles of polyamine metabolism during exposure to stress were studied by quantitative RT-PCR of genes involved in PA biosynthesis. For salt stress conditions comprehensive metabolic profiling was done for leaf and root samples to evaluate possible differences in metabolite levels. Hierarchical cluster analysis (HCA) was used to compare the metabolic changes during salinity acclimation in the generated tolerance classes. We furthermore investigated the possible presence of differential metabolite phenotypes under control conditions which may be indicative of salt tolerance or sensitivity. Multiple pool size changes for salt stress were established after analysis of the complete data set.
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1 - MPI of Molecular Plant Physiology, Am Mühlenberg 1, Potsdam, D-14424, Germany
2 - University of Stuttgart, Institute of Biology, Department of Botany, Pfaffenwaldring 57, D 70569 Stuttgart, Germany
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM