Mechanisms of Gene Regulation
Veley, Kira , Michaels, Scott D. .
Exploring the mechanisms by which the Autonomous Pathway functions in development.
Flowering marks a change from vegetative to reproductive growth in the shoot apical meristem. This decision is made using external factors such as day length and temperature, as well as internal signals via the autonomous floral-promotion pathway (AP). The AP consists of seven genes (FPA, LD, FLD, FVE, FCA, FY, and FLK), which all promote flowering by repressing the floral repressor, FLC; however, the molecular mechanism of this repression is largely unknown. In order to study the AP in greater detail, we made all possible double mutants among the seven members of the pathway and measured flowering time and vernalization responsiveness. Unexpectedly, we observed pleiotropic phenotypes in a subset of AP mutants crossed to fpa. Plants mutant for fpa and ld, fve, or fld display wavy leaves (i.e. cannot be flattened without cutting), a pale green rosette center, and reduced overall size and fertility. From these observations we conclude that these genes of the AP are involved in other aspects of development outside of flowering time and FLC repression. Next, we will investigate the differences among these plants at a molecular level. We hope to explain these phenotypes and, in turn, discover the molecular mechanism by which these members of the AP function.
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Michaels Lab website
1 - Indiana University, Biology, 915 E. 3rd Street, Bloomington, IN, 47405, United States
2 - Indiana University, Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM