sharma, sandeep .
Expression analysis of DREB related gene, TaDRF1, in two cultivars of bread hweat under water stress condition.
Adverse climatic conditions such as drought, low-temperature and high salinity cause a huge loss of grain yield every year globally. To improve the tolerance against these stress conditions, researchers emphasized on conventional breeding techniques along with biotechnological approaches.
Functional analysis revealed that transcription factors play a key role in the regulation of gene expression during abiotic stresses. DREB genes of Arabidopsis, were identified as important genes conferring drought, salinity and cold tolerance. In this study, we identified a DREB related gene, TaDRF1, through semi-quantitative RT-PCR, in two varieties of bread wheat, which expressed under water stress conditions. This gene belongs to AP2/ERF DNA binding domain family and produces three transcription factors TaDRF1.1, TaDRF1.2 and TaDRF1.3 through alternate splicing. This gene showed high homology with HvDRF1 gene of barley, expressed during dehydration.
We observed the expression profile of all the three transcripts of TaDRF1 gene in two varieties of bread wheat by quantitative RT-PCR. For this, seeds of both varieties C 306 and Bobwhite(write name of varieties) were sown under controlled condition in green house and leaf samples were collected from control, forth day stress and seventh day stress durations. Total RNA was isolated from all three samples and quantitative RT-PCR was carried out with TaqMan probes and primers. Our results revealed that all these transcripts showed different behavior in different time of water stress conditions. TdDRF1.1 and TDRF1.3 are transcriptional activators with AP2 domain and expressed in low quantity in controlled conditions but their quantity increased with stress duration. Where as, TdDRF1.2 is the most abundant and constant transcript under both the situations.
This TaDRF1 gene and its three transcripts could be used as indicators to select the desirable genotypes of bread wheat, which shows high degree of tolerance against hydric stress, and would help in molecular assisted breeding. The future application of the present finding lies in the generation and production of new bread wheat varieties (genotypes) drought-resistant, either by assisted breeding or by transgenesis.
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1 - banaras hindu university, Genetics and Plant Breeding, Room No. 135, BGT Hostel, BHU, Varanasi, Uttar pradesh, 221005, India
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM