Duceppe, Marc-Olivier , Cloutier, Conrad , Desjardins, Yves , Michaud, Dominique .
Differential effects of mechanical wounding and herbivory on the leaf proteome of potato, Solanum tuberosum.
A quick survey of the recent literature suggests the onset of differential responses to mechanical wounding and herbivory in leaves of vascular plants. To further document the specific effects of herbivory on the host plant metabolism, we compared the leaf proteome of potato plants (Solanum tuberosum L.) subjected to mechanical wounding [using a razor blade] or to herbivory by larvae of the Colorado potato beetle, Leptinotarsa decemlineata Say. Out of approximately 600 proteins detected on 2-D gels, 36 were significantly up- or down-regulated by one or both treatments. In particular, several proteins constituent of the chloroplast photosynthetic machinery were strongly down-regulated by the potato beetle treatment while the remaining were unaffected by wounding. Real-time RT-PCR analyses for the corresponding mRNA transcripts revealed similar down-regulating effects for the two treatments, consistent with the expected reallocation of energetic and metabolite resources towards the synthesis of defense-related compounds. The dissimilar responses observed at the proteome level suggest a specific, post-translational effect of the insect negatively altering the stability of some plant proteins. Western blot analyses using primary antibodies directed against different proteins of the photosynthetic apparatus were carried out to investigate further the observed protein degradation processes. A number of photosynthesis parameters were also monitored to determine how the down-regulation of specific photosynthetic proteins during herbivory impacts the host plant photosynthetic capacities.
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1 - University Laval, Phytology, Pavaillon INAF, Quebec, Quebec, G1K 7P4, Canada
2 - University Laval, Biology
3 - University Laval, Phytology
Potato (Solanum tuberosum)
Colorado potato beetle
Real time PCR.
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM