Choi, Suh-Yeon , Ilarslan, Hilal , Kourtz, Lauralynn , Snell, Kristi , Lawrence, Michael , Cook, Dianne , Nikolau, Basil J , Wurtele, Eve .
Transcriptomic and Metabolomic analysis of PHB-producing Arabidopsis.
Polyhydroxyalkanoates (PHAs) are a class of biodegradable polyesters (bioplastics) naturally produced by many bacteria. Production of PHAs in plants could provide a solar-energy-powered source of bioplastic. However, economically viable production of bioplastics in plants tends to have detrimental effects on growth, which limits the use of plants as bio-factories. To understand the relationship between carbon utilization and illumination cycles in the presence of a foreign sink for plastidic acetyl-CoA, we evaluated transgenic PHB-producing and wild type plants grown under three different illumination regimes. PHB accumulation was dependent on the light regime, and was as high as 15% of total dry weight. Metabolite profiles (non-targeted GC-MS analysis), the acyl-CoAs accumulation (LC/MS/MS), and transcriptomic profiles were determined. Transcriptomic and metabolomic data were evaluated using Explorase (MetNet Platform, http://metnet.vrac.iastate.edu) software to analyze and visualize omics data. Our working hypothesis is that that plant responds to a flux of carbon to PHB with transcriptional shifts that reallocate carbon in related pathways.
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MetNet platform to analyze and visualize omics data
1 - Iowa State University, Genetics, Development and Cell Biology, 433 Bessey Hall, Iowa State University, Ames, IA, 50011, USA
2 - Iowa State University, Genetics, Development and Cell Biology
3 - Metabolix Inc.
4 - Iowa State University, Statistics
5 - Iowa State University, Biochemistry, Biophysics, and Molecular Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM