Rhee, Hong Soon , Cho, Hwa-Young , Choy, Yoon-hi , Son, Seok Young , Park, Jeong Jin , Park, Jong moon .
Comparison of alkaloid production, gene transcripts in intact plants, suspended cell cultures and transgenic cells of Papaver somniferum .
Poppies are the only plant which can synthesize morphinan alkaloids including morphine, codeine, thebaine and a variety of other benzylisoquinoline alkaloids as secondary metabolites, but plant cells induced from same plant species have produced low level of or no morphinan alkaloids. The exact reason of the loss of morphinan alkaloids producing ability in plant cells have been elucidated yet but their changes of gene transcripts profiles during developmental process should be an important factor on changes of secondary metabolite synthesis. We investigated the difference metabolic production profiles between intact plants and suspended cells of Papaver somniferum and analyzed mRNA expression profiles of several genes on the mrphinan alkaloid biosynthetic pathway to find out that enzymes transforming (S)-reticuline, the critical intermetabolite which more than 3 pathways are branched from, toward sanguinarine, papaverine and morphine, that is berberine bridge enzyme(BBE), (R,S)-reticuline 7-O-methyl transferase(7OMT) and codeinone reductase(COR) respectively, were expressed differently affecting final secondary metabolites synthesis. Moreover, these enzymes could be the target genes for metabolic engineering of Papaver somniferum to produce high morphinan producing cell lines. We tried to over-express salutaridine reductase(SR), salutaridinol-7-O-acetyl transferase(SAT) and codeinone reductase(COR) on late biosynthetic steps of morphinans by genetic transformation of hypocotyls. Metabolite production profiles and transcript levels for nine enzymes in the pathway, both before and after (S)-reticuline, were analyzed by HPLC and real-time PCR.
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POSTECH Plant Secondary Metabolite Team
1 - POSTECH, Dept. Chem. Eng, Advanced Environmental Biotechnology Research Center, San 31, Hoyja-dong, Nam-gu, Pohang, Gyeongbuk, 790-784, South Korea
2 - POSTECH, School of Environmental Science and Engineering, Advanced Environmental Biotechnology Research Center
3 - Ehwa Woman's University, Dept. Life Science
4 - POSTECH, Dept. Chem. Eng, Advanced Environmental Biotechnology Research Center
5 - POSTECH, Dept. Chem. Eng, School of Environmental Science and Engineering, Advanced Environmental Biotechnology Research Center
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM