Mechanisms of Gene Regulation
Tefft, Nicholas , Miles, Timothy , Ransom-Hodgkins, Wendy .
Functional analysis of the Eukaryotic Elongation Factor One Alpha gene family.
Eukaryotic Elongation Factor One Alpha (eEF1A) is a multifunctional protein involved in protein synthesis and degradation, binding actin and micotubules, and several different signal transduction pathways in the cell. The regulation of eEF1A’s many functions is not understood. Arabidopsis thaliana has four eEF1A genes forming a small family. We have taken a loss of function approach to gain a better understanding of the importance and functions of each eEF1A family member. To accomplish this task we identified forty T-DNA knockout lines containing a T-DNA insertion in the promoter, coding, or 3’UTR region of each eEF1A gene. Several phenotypes including lethality, limited germination, delayed development, reduction in vitality, and loss in reproductive ability were observed in homozygous T-DNA lines for each eEF1A gene. RNA from wild type and T-DNA plants displaying unusual phenotypes was isolated for quantitative real time RT-PCR analysis. The quantitative real time RT-PCR data will be analyzed to determine changes in gene expression between the T-DNA and wild type plants. A model will be presented defining the importance or redundancy in function of each protein within the family. Support provided by NSF DBI0552517 for NT.
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1 - Western Michigan University, Biological Sciences
2 - Michigan State University, Plant Pathology
3 - Western Michigan University, Biological Sciences, 3441 Wood Hall, 1903 West Michigan Ave., Kalamazoo, MI, 49008-5410, USA
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM