Romero, M Carmen , Brown, Daniel , Gijzen, Mark .
Proteome analysis of soybean developing seed coats.
The relatively large seeds in soybean (Glycine max) make it a good legume model to study seed coat development. Soybean seed coats are an agricultural by-product that could be further utilized to produce high value proteins and augment the crop market value. Many seed coat proteins have been previously described and analyzed, but a comprehensive proteome analysis of seed coat tissues has not yet been reported. The identification of proteins and their allocation to the different seed coat maternal tissues (epidermis, hourglass cells and parenchyma) and filial tissue (aleurone) could provide insight to the seed coat function in the regulation of embryo development, seed biology, and open the possibility for manipulation of seed quality features. We report on a proteomic approach used to determine the protein expression profile of seed coats. Seed coat extracts were analyzed by 2D gels at different developmental stages. In the early stage (10-20 days post-anthesis, DPA), the seed coat represents at least 87 % of the seed mass and cell division is predominant, circa 700 spots were detected. In the mid stage of development (21-35 DPA) circa 800 spots were detected, corresponding to the elongation phase of seed development. In the late stage of development (36-50 DPA) circa 400 spots represent the accumulation of storage proteins as the seeds enter maturation. Two-dimensional maps of cv Harosoy 63 were analyzed by Image Master 2D Platinum 6.0 software. The expected dramatic changes in seed coat protein expression profile associated with the major developmental stages of the soybean seed are documented. Selected highly abundant spots were analyzed by MALDI-TOF. Future work aims at elucidating key potential protein markers and potential developmental regulators.
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1 - University of Western Ontario, Department of Biology, Biological & Geological Sciences Building, London, Ontario, N6A 5B7, Canada
2 - Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, London, ON, N5V 4T3 Canada
seed coat proteome
legume seed coat.
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM