Protein Targeting and Vesicular Trafficking
Kuppu, Sundaram , Venkataramani, Sujatha , Zhang, Hong .
The role of AKR2 in the biogenesis of some membrane proteins.
Protein-protein interaction plays an important role in controlling protein stability, protein targeting, and signal transduction. Stabilizing and targeting of membrane-bound proteins to their destinations are vital for plant growth and development. Therefore, understanding the mechanism of stabilization and targeting of these membrane-bound proteins is very important. Earlier research in our laboratory had shown that ankyrin repeat-containing protein 2 (AKR2) interacts with the peroxisomal membrane-bound ascorbate peroxidase 3 (APX3). AKR2 also interacts with other membrane-bound proteins such as cytochrome b5 (CB5), cytochrome b5 reducates (CB5R) and the chloroplast outer envelop proteins OEP7. The AKR2-interaction sites in these membrane proteins are their transmembrane domains. Consequently, we hypothesized that AKR2 might regulate the biogenesis of these membrane proteins. To test our hypothesis we are  studying the role of AKR2 in plant growth and development by analyzing AKR2 down-expression lines (via RNA interference) and  analyzing the localization and behavior of GFP-APX3 fusion proteins in AKR2 down-expression lines (including RNA interference lines, AKR2 tilling mutants, and AKR2 antisense lines). This research will lead to a better understanding of the role that AKR2 plays in the biogenesis of a group of membrane-bound proteins and elucidating a mechanism about the roles that AKR2 plays in plant growth and development, disease resistance, and antioxidation metabolism at molecular level. Most recent data on the characterization of AKR2 down-expression lines will be presented at the meeting.
Log in to add this item to your schedule
1 - Texas Tech University, Biological Sciences, pobox 43131, Lubbock, Texas, 79409, United States of America
2 - The University of Chicago, Molecular Genetics & Cell Biology
3 - Texas Tech University, Biological Sciences
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM