Growth and Vegetative Development
Teotia, Sachin , Rebecca, Lamb .
Two putative Poly (ADP-ribose) polymerases, RCD1 and SRO1, play important, partially redundant roles in Arabidopsis development.
RADICAL-INDUCED CELL DEATH1 (RCD1) has been identified as a stress response gene, interacting with several stress and hormone response pathways. RCD1 consists of a poly(ADP-ribose) polymerase (PARP) domain and a WWE protein-protein interaction domain. PARPs mediate attachment of ADP-ribose units from donor NAD+ molecules to target proteins and have been implicated in a number of processes including DNA repair, apoptosis, transcription, and chromatin remodeling. RCD1 and SIMILAR TO RCD ONE1 (SRO1) are the only two proteins encoded in the Arabidopsis genome containing both WWE and PARP domains; similar proteins have been found in all groups of eukaryotes. Like RCD1, SRO1 is expressed in all plant tissues examined. We have isolated homozygous null mutants in both RCD1 and SRO1, rcd1-3 and sro1-1, respectively. rcd1-3 plants display similar phenotypic defects to those reported for previously isolated alleles (rcd1-1 and rcd1-2), like reduced stature, malformed leaves and early flowering. In addition, the plants have abnormal phyllotaxy, small, deformed floral organs, increased lateral root number and length, and shorter primary root. sro1-1 plants display some subtle developmental defects similar to the root and flower phenotypes of rcd1-3. However, plant height, leaf shape and size, and flowering time appear normal. Preliminary analysis of stress response indicates that SRO1 may have a different function than RCD1 in these pathways. Double mutant plants of rcd1-3;sro1-1 display severe developmental defects including germination defects, extreme dwarfism, abnormal flowers, and short siliques. The double mutant phenotype suggests that RCD1 and SRO1 are at least partially redundant with one another and that they are essential genes for plant development.
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1 - The Ohio State University, Plant Cellular & Molecular Biology, 500, Aronoff Laboratory, 318- West 12th Avenue, Columbus, OHIO, 43210, U.S.A.
2 - The Ohio State Univeristy, Plant Cellular and Molecular Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM