Chaikam, Vijay , Karlson, Dale, T .
Stress and developmental expression of cold shock domain proteins in rice.
Cold shock domain proteins (CSPs) are the most ancient and well conserved group of proteins that are present in nearly all living organisms. In bacteria, cold shock domain proteins play critical roles in their adaptation to cold by acting as RNA chaperones and transcription anti-terminators. Cold shock domain proteins from animal systems are known for various roles in development. At the present time, the function of plant cold shock domain proteins is not well understood. Previous expression analyses of CSPs from winter wheat and Arabidopsis confirmed an up-regulation of gene expression upon cold treatment, suggesting a correlative role in the cold adaptation of these plants. Unlike Arabidopsis and wheat, rice is unable to cold acclimate and thereby presents us with a comparative model system to study the functional role of CSPs in relation to low temperature stress. In the present study, two cold shock domain proteins were cloned from rice (RCSP1 and RCSP2). Similar to wheat and Arabidopsis, rice RCSPs complemented a cold sensitive E. coli strain which lacks four cold shock domain proteins and both exhibited ssDNA binding activity. Unlike winter wheat and Arabidopsis, RCSP transcripts increased only transiently and marginally when exposed to low temperature stress. Specifically, RCSP transcripts increased to maximum levels 30 min subsequent to cold stress and decreased steadily thereafter. Expression analyses from a developmental time-course revealed that both RCSP1 and RCSP2 are expressed at high levels in tissues with meristematic activity. Comparative protein blot analyses with a wheat cold shock domain specific antibody provided data in good accordance to expression data obtained from both the cold stress and developmental studies. These correlative data suggest that RCSPs may function in a development role for rice.
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1 - West Virginia University, Division of Plant and Soil Sciences, 1090 Agriculture Sciences Building, Morgantown, WV, 26506-6108, USA
2 - West Virginia University, Division of Plant and Soil Sciences
cold shock domain
low temperature stress
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM