Rao, Hongyu , Hirsch, Cory D , Kim, Joonyup , Carpita, Nicholas C , McCann, Maureen , Willats, William G T , Patterson, Sara E. .
Characterization of an ectopic cell separation mutant tfa1-2 "things fall apart".
Plant cell walls are essential for basic plant structure and often thought of as rigid and inflexible; however, walls are dynamic, responding to developmental and environmental cues. Examples of developmental regulation of cell to cell adhesion or cell separation processes include lateral root development, hypocotyl elongation, abscission and dehiscence. Unfortunately, it is still unclear as to how plants integrate the processes of cell to cell adhesion and plant cell separation with these developmental and environmental cues. We have identified several ectopic cell separation mutants that we have designated tfa "things fall apart." These mutants are characterized by unregulated cell separation in epidermal, cortical and vascular tissues. This phenotype is most severe in young seedlings affecting the hypocotyl, cotyledon, and first true leaves. Many of the young seedlings undergoing this irregular ectopic cell separation do not develop normal root and shoot systems and eventually die when cultured on the 1/2MS media with 0.8% agar, at 23?C. Further characterization of tfa1-2 indicates that the defective cell adhesion in tfa1-2 young seedlings can be partially recovered by culturing tfa1-2 seeds on the 1/2 MS media with changes in the percentage of agar or altered temperatures. Under these conditions, tfa1-2 will develop normally and can be successfully transplanted and grown to maturity. Using RTPCR and immunolocalization, we have observed differential expression of several cell wall associated genes in mutant versus wild type seedlings. In addition, cell wall analyses of monosaccharide distribution in mutant and wild type will also be presented. tfa1-2 was identified from an EMS screen of Arabidopsis seedlings and PCR-based mapping indicates that it is a putative methyltransferase At1g78240. We will present the phenotypic, physiological and molecular characterization of tfa1-2 and hypotheses for potential role of TFA1 during development.
This work was funded by USDA grant 0035301-9085 and NSF DBI-0077719.
*Corresponding author: email@example.com
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Patterson web site
Purdue Cell Wall web site
1 - University of Wisconsin, Madison, Department of Horticulture
2 - Purdue University, West Lafayette, Indiana, Department of Botany and Plant Pathology
3 - Purdue University, West Lafayette, Indiana, Department of Biological Sciences
4 - University of Copenhagen, Copenhagen, Denmark, Institute of Molecular Biology and Physiology
5 - University of Wisconsin, Madison, Department of Horticulture, 1575 Linden Dr, Madison, WI, 53706, USA
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM