Unable to connect to database - 02:36:52 Unable to connect to database - 02:36:52 SQL Statement is null or not a SELECT - 02:36:52 SQL Statement is null or not a DELETE - 02:36:52 Botany & Plant Biology 2007 - Abstract Search
Unable to connect to database - 02:36:52 Unable to connect to database - 02:36:52 SQL Statement is null or not a SELECT - 02:36:52

Abstract Detail


Metabolism

Wang, Hongyun [1], Lin, Yun [2].

Developmental control of Arabidopsis seed oil biosynthesis.

Arabidopsis transcriptional factors LEAFY COTYLEDON1 (LEC1), LEAFY COTYLEDON2 (LEC2), FUSCA3 (FUS3), ABSCISIC ACID3 (ABI3), and ABSCISIC ACID5 (ABI5) are known to regulate multiple aspects of seed development. In an attempt to understand the developmental control of storage product accumulation, we observed the expression time course of the five transcripts. The sequential expression of these factors during seed fill suggests differentiation of their normal responsibilities. By extending the expression periods of the two early genes LEC1 and LEC2 in transgenic seeds, we demonstrated that the subsequent timing of FUS3, ABI3, and ABI5 transcripts depends on LEC1 and LEC2. Because a delayed onset or reduced level of FUS3 mRNA coincide with reduction of seed oil content in the transgenic seeds, the role of FUS3 in oil deposition was further examined. Analysis of published seed transcriptome data indicated that FUS3 transcript increases together with nearly all the plastidial fatty acid biosynthetic transcripts during development. The ability of FUS3 to induce fatty acid biosynthetic gene expression was further confirmed in transgenic Arabidopsis seedlings expressing a dexamethasone-inducible FUS3 cDNA. By accommodating the current evidence, we propose a hierarchical architecture of the transcriptional network in Arabidopsis seeds in which the oil biosynthetic pathway is integrated through the master transcriptional factor FUS3. Further examination of the underlying molecular mechanisms using transient gene expression assay will also be presented.


Log in to add this item to your schedule

1 - University of Illinois at Urbana-Champaign, Department of Crop Sciences, 1101 Peabody Dr, 184 National Soybean Research Center, Urbana, IL, 61801, USA
2 - University of Illinois at Urbana-Champaign, Department of Crop Sciences

Keywords:
Seed
oil
fatty acid
microarray.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P19011
Abstract ID:474


Copyright 2000-2007, Botanical Society of America. All rights