Thompson, Kari, B , Hasebe, Mitsuyasu , Karlson, Dale, T .
Composition and Evolution of the Cold Shock Domain Proteins in Plants.
Cold shock domain proteins (CSPs) are among the most ancient and conserved nucleic acid binding proteins. CSPs are predicted to have evolved prior to the divergence of prokaryotes and eukaryotes. Although they have been well characterized in both prokaryotes and eukaryotes, little is known about their evolutionary history and function in plants. CSPs can be identified in almost all plants ranging from various algal species through angiosperms. In order to demonstrate their highly conserved nature, a recent publication aligned 25 orthologues of plant CSPs from various members within the plant kingdom. We expanded upon the previous study by gathering additional ESTs (expressed sequence tags) with the BLAST (Basic Local Alignment Search Tool) tool from numerous public databases. Eighty-two additional unique CSP sequences were found from plants spanning a wider evolutionary history. These sequences were aligned and analyzed with ClustalX in order to develop a nomenclature formula for naming the various motifs contained within plants CSPs. To gain insight into the basic evolutionary history of the cold shock domain, these CSP sequences were used to generate phylogenetic trees using the PHYLIP program. Trees that were generated with this program include: Neighbor-Joining, Most Parsimonious, and Maximum Likelihood trees. Interestingly, the monocot and dicot CSPs deviate in the phylogenetic trees and can be further broken into subgroups. The monocots have two major types and the dicots have three major types of CSPs. In ancestral plant models, such as gymnosperms, their CSP sequences occupy their own branches. All of the CSPs show similarity in their N-terminal domains to CspA, the major cold related CSP from E.coli. The kingdom Plantae demonstrates a striking conservation of CSPs throughout their evolutionary history.
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1 - West Virginia University, Division of Plant and Soil Sciences
2 - National Institute for Basic Biology, Division of Evolutionary Biology
3 - West Virginia University, Division of Plant and Soil Sciences, 1090 Agriculture Sciences Building, Morgantown, WV, 26506-6108, USA
cold shock domain
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM