Das, Sampa , Saha, Prasenjit , Dasgupta, Indranil .
Transgenic lectin rice plants showing resistance to rice tungro disease.
Rice production is severely affected by viral diseases transmitted by homopteran group of rice pests, brown planthopper (BPH) and green leafhopper (GLH) feeding by phloem abstraction. Allium sativum leaf agglutinin ( ASAL) isolated from leaves of garlic was previously found to have detrimental effect on survival and multiplication of above two insects, administered in artificial diet as well as in ASAL expressing transgenic plants. ASAL was targeted to the insect feeding site of the elite cultivar of rice, susceptible to hemipteran pests under the control of phloem-specific Agrobacterium rolC and rice sucrose synthase-1 (RSs1) promoters. PCR, Southern blot and C-PRINS analyses of transgenic plants have confirmed stable T-DNA integration and the transgenes were observed to co-segregate among self-fertilised progenies. The single copy transgene bearing T0 and T1 plants with intact T-DNA expression cassette, exhibited stable expression of ASAL in northern and western blot analyses. ELISA determined the level of expressed ASAL up to the level of 1.01% of total soluble protein in some selected lines. Immunohistofluorescence localization of ASAL in those lines documented the desired pattern of expression regulated by each promoter type. In-planta bioassay studies revealed that transgenic ASAL adversely affect survival, growth and population of BPH and GLH. GLH resistant T1 plants were further evaluated for the incidence of tungro disease, caused by co-infection of GLH vectored Rice tungro bacilliform virus (RTBV) and Rice tungro spherical virus (RTSV). The results exhibiting minimization of disease infestation by RTBV/RTSV infection mediated by GLH, fed on ASAL expressing transgenic rice plant will be presented.
Saha etal : Planta 223: 1329-1343,2006
Saha et al : PMB 62:735-752, 2006
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1 - Bose Institute, PMCG, P 1/12 CIT Scheme VII-M,, Kolkata, 700054, India
2 - Bose Institute, PMCG
3 - Delhi University, South Campus, Plant Molecular Biology
Rice tungro disease
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM