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Abstract Detail


Metabolism

Gonzalez-Jorge, Sabrina [1], Mehrshahi, Payam [2], Bennett, Malcolm J. [2].

Dissecting the genetic control of folate homeostasis in plants.

Folates act as essential cofactors in the one-carbon metabolism in cells, which are important for DNA replication and division, de novo synthesis of pyrimidines, purines, methionine, histidine and serine. Unlike plants, mammals and humans cannot synthesise their own folate and therefore require a dietary source of folate, enhanced by fortification or supplementation. As folate deficiency is endemic to both developing and developed countries, a growing international interest and research has been directed towards enhancing folate abundance. We have probed the mechanisms controlling folate homeostasis in plants by selecting Arabidopsis lines with altered sensitivity towards the antifolate, methotrexate (MTX). We demonstrate that resistance results from reduced polyglutamation which is known to enhance MTX efflux from cells (Ravanel et al., 2001). MTX resistance could also result from reduced cellular uptake. Methotrexate resistance screens were carried out on a mutagenised Arabidopsis population and the Ler/Cvi near-isogenic line (NIL) population. We will report our latest results.
Ravanel S., Cherest H., Jabrin S., Grunwald D., Surdin-Kerjan Y., Douce R., and Rébeillé F. (2001) Tetrahydrofolate biosynthesis in plants: Molecular and functional characterization of dihydrofolate synthetase and three isoforms of folylpolyglutamate synthetase in Arabidopsis thaliana PNAS 98 (26): 15360-15365


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Bennett Laboratory Research Interests


1 - University of Nottingham, Plant Sciences Division, School of Biosciences, Sutton Bonington Campus, Loughborough, Leicestershire, LE12 5RD, United Kingdom
2 - University of Nottingham, Plant Sciences Division

Keywords:
Folate homeostasis
Genetic screens
Folate analogs.

Presentation Type: Plant Biology Abstract
Session: P
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM
Number: P19017
Abstract ID:467


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