Feng, Teng-Yung , Huang, Hsiang-En .
Ferredoxin-1 involvement in the regulation of plant innate immunity via production of active oxygen species in Arabidopsis.
Ferredoxin-1 (Fd1) that functions in the catalysis of electron flow is generally involved in the production of active oxygen species (AOS) and plant disease resistance. Previously, the disease immunity of various transgenic plants was enhanced by the over-expression of sweet pepper Fd1 protein (PFLP)(see note 1). In this communication the pflp gene is transferred to wild-type and resistance-defective Arabidopsis to reveal the mechanism of Fd1-mediated resistance. The wild-type plant over-expression PFLP is resistant to Erwinia carotovora pv. carotovora (ECC). This resistance is abolished by the presence of AOS inhibitor and protease inhibitors. Under the conditions of Erwinia infection, the production of AOS and the activation of NADP oxidase in the pflp-transgenic plant are increased by the treatment of salicylic acid (SA), harpin or Pseudomonas syringae pv.syringae . In contrast, it is reduced by jasmonic acid and ECC. The alterations of Fd1 in pathogen-inoculated plants are observed in all wild-type, eds1, and pad4 mutant Arabidposis, but not in nahG transgenic and npr1 mutant. Over-expressed PFLP in nahG transgenic and npr1 mutant are able to increase disease resistance to ECC, but not in eds1 mutant. These results imply that Fd1-mediated disease resistance might be downstream of SA and npr1 but independent with eds1.
Note 1: reference cited
1. Plant Sci. 2001, 160:1035;
2. Plant Mol. Biol. 2003, 51:913;
3. Transgenic Res. 2004, 13:567;
4. Mol. Plant Pathol. 2007, 8:129.
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Dr. Feng's work
1 - Academia Sinica, Plant and Microbial Biology, Acadmia Road, Section 2, Nankang, Taipei, Taiwan, 11529, Taiwan
2 - Academia Sinica, Plant and Microbial Biology
Presentation Type: Plant Biology Abstract
Location: Exhibit Hall (Northeast, Southwest & Southeast)/Hilton
Date: Sunday, July 8th, 2007
Time: 8:00 AM